Promega pmirGLO Brings New Capability for Study of microRNA Activity
MADISON, WISCONSIN (February 26, 2009)
Promega Corporation announces the availability of the new pmirGLO Dual-Luciferase miRNA Target Expression Vector. pmirGLO supports both breadth and depth in the microRNA study. Researchers have both ability to measure a wide dynamic range of miRNA activity in a vast number of cell types and the option to create a stable cell line from one genetic construct for further research. More specifically, pmirGLO uses optimized firefly luciferase gene as the readout for activity, while also offering a Renilla luciferase/neomycin resistance gene for data normalization.
The pmirGLO vector allows a luciferase reporter gene assay to measure the function of endogenous or exogenous micro RNAs (miRNA). This vector also enables functional studies of sequence variations found in 3'-untranslated regions of genes often related to miRNA binding sites or other modulators of transcript stability. These elements are cloned into the pmirGLO vector to create a transcriptional fusion to firefly luciferase, allowing quantitative results with a simple and sensitive assay. This vector is the most recent addition to a collection of nearly 50 luciferase reporter vectors offered by Promega.
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