Sample Types Processed with the Wizard® SV Genomic DNA Purification System
DNA from the following sample types has been successfully purified at Promega or by external laboratories using the Wizard® SV Genomic DNA Purification System (Cat.# A2360, A2361).
Sample Types
Many of these protocols have been communicated to Promega by external laboratories. Because these protocols have not been performed at Promega, they are intended to be guidelines that might require optimization.
This list will be updated as additional information becomes available. Contact Promega Technical Services for the latest information on available protocols.
Sample Types Processed Using the Wizard® SV Genomic DNA Purification System.
| Sample Type |
Promega |
External |
Comments |
| Blood and blood cells |
|
|
|
| Whole blood |
|
Yes |
Researchers isolated bacterial DNA from whole blood samples inoculated with Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae. Smith, K., Diggle, M.A. and Clarke, S.C. (2003) J. Clin. Microbiol. 41, 2440–3.
PubMed ID #12791861. |
| Peripheral blood mononuclear cells |
|
Yes |
Ezzeldin, H.H. et al. (2005) Clin. Cancer Res. 11, 8699–705.
PubMed ID #16361556. |
| Mouse primary granulocytes, macrophages, B lymphocytes and T lymphocytes |
|
Yes |
Morii, E. et al. (2004) Blood 104, 1656–61.
PubMed ID #15172970. |
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| Sample Type |
Promega |
External |
Comments |
| Mammalian tissues |
|
|
|
| Mouse tail clipping |
Yes |
Yes |
A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
Reference:
Smith, P. et al. (2005) J. Exp. Med. 202, 1319–25.
PubMed ID #16301741. |
| Mouse liver |
Yes |
|
A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302. |
| Mouse brain |
Yes |
|
A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302. |
| Mouse heart |
Yes |
|
A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302. |
| Mouse spleen |
|
Yes |
Fakhari, F.D. et al. (2006) J. Clin. Invest. 116, 735–42.
PubMed ID #16498502. |
| Mouse ear |
|
Yes |
Du, Q. et al. (2006) FASEB J. 20, 1131–41.
PubMed ID #16770012. |
| Mouse skin |
|
Yes |
Basal cell carcinoma and noncancerous tissues were frozen at –80°C prior to DNA isolation.
Mancuso, M. et al. (2006) Cancer Res. 66, 6606–14.
PubMed ID #16818633. |
| Anopheles funestus mosquito carcass |
|
Yes |
Michel, A.P. et al. (2006) Genetics 173, 1389–95.
PubMed ID #16648581. |
| Human lung cancer biopsy |
|
Yes |
Sasaki, H. et al. (2005) Clin. Cancer Res. 11, 2924–9.
PubMed ID #15837743. |
| Human distant normal colorectal mucosa and tumor tissue |
|
Yes |
Pfeifer, D. et al. (2005) Carcinogenesis 26, 103–7.
PubMed ID #15485994. |
| Human frozen thymic epithelial tumor samples |
|
Yes |
Suzuki, E. et al. (2006) Jpn. J. Clin. Oncol. 36, 351–6.
PubMed ID #16762968. |
| Human pancreatic islet |
|
Yes |
Del Guerra, S. et al. (2005) Diabetes 54, 727–35.
PubMed ID #15734849. |
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| Sample Type |
Promega |
External |
Comments |
Cultured cells
(Established cell lines are not listed.) |
Yes |
|
A protocol for DNA isolation from mammalian cultured cells is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302. Any additional resources are listed below. |
| Chicken retinospheroid cultures |
|
Yes |
Paraoanu, L.E. et al. (2006) Toxicol. Sci. 89, 314–24.
PubMed ID #16207942. |
| HT1080 human fibrosarcoma |
|
Yes |
Cells were transfected with FIV vectors, and genomic DNA was isolated 3 days post-transduction.
Harper, S.Q. et al. (2006) J. Virol. 80, 9371–80.
PubMed ID #16973543. |
| Monkey primary fibroblasts, virus-infected |
|
Yes |
Cells were infected with ~1 multiplicity of infection (MOI) of pseudotyped HCV and total DNA isolated.
Blanchard, E. et al. (2006) J. Virol. 80, 6964–72.
PubMed ID #16809302. |
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| Sample Type |
Promega |
External |
Comments |
| Bacteria |
|
|
|
| Bacillus macyae |
|
Yes |
Cells in mid- to late exponential phase were centrifuged for 20 minutes at 21,000 × g at 4°C.
Santini, J.M. et al. (2004) Int. J. Syst. Evol. Microbiol. 54, 2241–4.
PubMed ID #15545465. |
| E. coli K-12 derivative, MC4100 |
|
Yes |
Teresa Pellicer, M. et al. (2003) J. Bacteriol. 185, 5815–21.
PubMed ID #13129953. |
| Pseudomonas aeruginosa |
|
Yes |
Zhu, H. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45, 1897–903.
PubMed ID #15161855. |
| Bacillus subtilis |
|
Yes |
Park, J.H. et al. (2004) J. Bacteriol. 186, 1571–3.
PubMed ID #14973012. |
| Eleven Gram-negative bacterial species |
|
Yes |
Modified protocol using the Wizard® SV Genomic Purification System to isolate bacteria as follows: The pelleted bacteria were resuspended in 400µl of enzymatic lysis solution (47mM EDTA, 25mg/ml lysozyme, 20µg/ml lysostaphin) and incubated for 2 hours at 37°C. Next, 19.2 mg/ml proteinase K was added (final concentration 0.4 mg/ml), and the mixture was incubated for 1 hour at 55°C. Finally, the Nuclei Lysis Solution and RNase Solution were added, mixed and incubated for 10 minutes at 80°C.
Zucol, F. et al. (2006) J. Clin. Microbiol. 44, 2750–9.
PubMed ID #16891488. |
| Eleven Gram-positive bacterial species |
|
Yes |
Modified protocol using the Wizard® SV Genomic Purification System to isolate bacteria as follows: The pelleted bacteria were resuspended in 400µl of enzymatic lysis solution (47mM EDTA, 25mg/ml lysozyme, 20µg/ml lysostaphin) and incubated for 2 hours at 37°C. Next, 19.2mg/ml proteinase K was added (final concentration 0.4mg/ml), and the mixture was incubated for 1 hour at 55°C. Finally, the Nuclei Lysis Solution and RNase Solution were added, mixed and incubated for 10 minutes at 80°C.
Zucol, F. et al. (2006) J. Clin. Microbiol. 44, 2750–9.
PubMed ID #16891488. |
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| Sample Type |
Promega |
External |
Comments |
| Other organisms and tissues |
|
|
|
| Schistosoma mansoni (blood fluke) worm |
|
Yes |
Smith, P. et al. (2004) J. Immunol. 173, 1240–8.
PubMed ID #15240716. |
| Snail cercariae |
|
Yes |
Smith, P. et al. (2004) J. Immunol. 173, 1240–8.
PubMed ID #15240716. |
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