Rabbit Reticulocyte Lysate System, Nuclease Treated

Rabbit Reticulocyte Lysate Translation Systems are utilized in the identification of mRNA species, the characterization of their protein products and the investigation of transcriptional and translational control. Rabbit Reticulocyte Lysate is prepared from New Zealand white rabbits using a standard protocol that ensures reliable and consistent reticulocyte production in each lot. After the reticulocytes are lysed, the extract is treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum. The lysate contains the cellular components necessary for protein synthes...

Expand to Read More »

Rabbit Reticulocyte Lysate Translation Systems are utilized in the identification of mRNA species, the characterization of their protein products and the investigation of transcriptional and translational control. Rabbit Reticulocyte Lysate is prepared from New Zealand white rabbits using a standard protocol that ensures reliable and consistent reticulocyte production in each lot. After the reticulocytes are lysed, the extract is treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum. The lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors).

Features - Benefits

• Consistent: Reliable and consistent translation with each lot.
• Optimized and Ready to Use: The treated Rabbit Reticulocyte Lysate is optimized for translation and contains an energy-regenerating system (phosphocreatine/phosphocreatine kinase), a mixture of tRNAs (to expand the range of mRNAs that can be translated), hemin (to prevent inhibition of initiation), and potassium chloride and magnesium acetate.
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/

Applications

• In vitro translation from mRNA, in vitro run-off transcripts or total RNA into protein.
• Characterization of mRNA products.
• Co-translational processing of secreted proteins by the addition of microsomal membranes to the translation reaction.

For more information, see the Protein Expression chapter of the Protocols & Applications Guide.

References

1. Pelham, H.R.B. and Jackson, R.J. (1976) Eur. J. Biochem. 67, 247–56.
2. Glass, C.A. and Pollard, K.M. (1990) Promega Notes 26, 6–9.

« Collapse Content