Optimized Gene Expression with the T7 Sample System

The T7 Sample System contains four unique in vitro translation systems for one-step gene expression from circular and linear plasmid DNA templates, as well as nonpurified PCR products. The system contains samples of TnT® T7 Quick for PCR DNA, TnT® T7 Quick Coupled Transcription/Translation System, TnT® T7 Coupled Wheat Germ Extract System and the E. coli T7 S30 Extract System for Circular DNA. This article discusses the influence of vector and insert sequences on optimal gene expression in both eukaryotic and prokaryotic in vitro translation systems as shown using these four gene expression systems.

Promega Notes 80, 10–13.