E. coli T7 S30 Extract System for Circular DNA Technical Bulletin
Literature # TB219
The E. coli T7 S30 Extract System for Circular DNA simplifies transcription/translation of DNA sequences cloned in plasmid or lambda vectors containing a T7 promoter by providing an extract that contains T7 RNA polymerase for transcription and all necessary components for translation. The investigator supplies only cloned DNA containing a T7 promoter and a ribosome binding site. The T7 S30 Extract for Circular DNA is prepared by modifications of the method described by Zubay from an E. coli strain B deficient in OmpT endoproteinase and lon protease activity. This results in greater stability of expressed proteins that would otherwise be degraded by proteases if expressed in vivo.
Printed in USA. Revised 3/09.