CytoTox-Glo™ Cytotoxicity Assay Technical Bulletin

Literature # TB359

The CytoTox-Glo™ Cytotoxicity Assay uses a luminogenic peptide substrate, the AAF-Glo™ Substrate, to measure dead-cell protease activity, which is released from cells that have lost membrane integrity. The AAF-Glo™ Substrate cannot cross the intact membrane of live cells and does not generate any appreciable signal from the live-cell population. The CytoTox-Glo™ Assay relies on the properties of Ultra-Glo™ Recombinant Luciferase, which uses aminoluciferin as a substrate to generate a stable "glow-type" luminescent signal and is formulated to improve performance across a wide range of assay conditions.