Promega Corporation

MagneGST™ Protein Purification System Technical Manual

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Literature # TM240

The MagneGST™ Protein Purification System provides a simple, rapid and reliable method for the purification of glutathione-S-transferase (GST) fusion proteins. Glutathione immobilized on paramagnetic particles (MagneGST™ Glutathione Particles) is used to isolate GST-fusion proteins directly from a crude cell lysate using a manual or automated procedure. The use of paramagnetic particles eliminates several centrifugation steps and the need for multiple tubes. It also minimizes the loss of sample material. Although the MagneGST™ System is designed for manual applications, samples can also be processed using a robotic platform, such as the Beckman Coulter Biomek® 2000 or Biomek® FX workstation, for high-throughput applications.

Printed in USA. Revised 12/13.

 

PDF Icon Complete Protocol PDF (1226kb)

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