rProteinase K Solution AOF
Animal-Origin-Free Recombinant Proteinase K
- Active over pH range 4.3–12.0 in 0.5% SDS or 1% Triton® X-100
- Retains >80% activity at temperatures up to 60°C
- No resuspension or thawing before use
- Manufactured under cGMP for consistent, lot-to-lot performance
- Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated
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Catalog Number: A5055
Recombinant Proteinase K for Diagnostic and Therapeutic Development
Recombinant Proteinase K, based on the enzyme from the fungus Tritirachium album Limber, is a serine protease that exhibits broad cleavage activity. It cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids and is useful for general digestion of protein in biological samples.
Recombinant expression of Proteinase K reduces risk of contamination, which when combined with our stringent quality system, ensures the high level of purity and consistency required by diagnostic and therapeutic developers.
The stability of Proteinase K in urea and SDS and its ability to digest native proteins make it useful for a variety of applications including preparation of chromosomal DNA for pulsed-field gel electrophoresis, protein fingerprinting and removal of nucleases from preparations of DNA and RNA. A typical working concentration for Proteinase K is 50–100μg/ml.
Learn more about our Animal-Origin-Free reagents and comprehensive quality system.
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Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
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