PowerPlex® ESX 17 and ESI 17 Pro Systems
Co-Amplification and 5-Color Detection of 17 Loci
- Amplify all ENFSI-recommended loci, including SE33
- Obtain more complete profiles from degraded DNA
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Catalog Number: DC6720
Catalog Number: DC6794
Catalog Number: DC7780
Analyze Challenging Samples and Confirm Your Results
The PowerPlex® ESX 17 and ESI Pro Systems were developed for human identification applications including forensic analysis, relationship testing and research use and meet the new ENFSI recommendations for DNA profile sharing across Europe. They are based on five-color technology and allow co-amplification and detection of 17 loci, including D18S51, D21S11, TH01, D3S1358, Amelogenin, D16S539, D2S1338, D1S1656, D10S1248, FGA, D8S1179, vWA, D22S1045, D19S433, D12S391, D2S441 and SE33.
These two STR systems are available separately or as a bundle and can be used to complement each other when analyzing degraded or challenging samples to maximize recovery of allelic information from as many loci as possible and also allow confirmation of results obtained with the other system.
These systems include a proprietary 5X master mix that contains hot-start Taq DNA polymerase, buffer, salts, MgCl2 and dNTPs. Additionally, the kits have increased tolerance to common inhibitors and increased sensitivity to obtain full profiles from low-level DNA and are robust enough to genotype degraded DNA samples through the use of mini-STR loci.
The PowerPlex® ESX 17 and ESI 17 Pro Systems are compatible with Spectrum Compact CE System and Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. Panels and bins text files are required for use with GeneMapper® ID and ID-X software and are available for download.
PowerPlex® ESX 17 System
The PowerPlex® ESX 17 System amplifies the loci recommended by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP) as mini-STRs (<125bp; D2S441, D10S1248 and D22S1045) or midi-STRs (125–185bp; D1S1656 and D12S391).Â
PowerPlex® ESI 17 Pro System
The PowerPlex® ESI 17 Pro System is designed to amplify six of the original seven European Standard Set (ESS) loci (D3S1358, D18S51, TH01, vWA, D8S1179 and the more common FGA alleles) along with D16S539 and D19S433 as smaller amplicons (<250bp), while the loci recommended by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP) (D1S1656, D2S441, D10S1248, D12S391 and D22S1045) are present as larger amplicons.
The PowerPlex® ESI 17 Pro System is an update to the PowerPlex® ESI 17 System. All primer pairs, with the exception of the SE33 primer pairs, are identical in both systems. The internal lane standard, WEN Internal Lane Standard 500 ESS, is designed and optimized specifically for this system.
Rapid Processing Solutions for PowerPlex® Systems
The 5X AmpSolution™ Reagent, SwabSolution™ Kit and PunchSolution™ Kit allow fast and simple processing of swabs and punches for analysis with various PowerPlex® Systems, including the PowerPlex® Fusion, 21, 18D, 16 HS, ESX, ESI, CS7 and Y23 Systems. These products are intended for preparation of single-source reference, database and paternity samples where sample purification is unnecessary.
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Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
2800M Control DNA |
DD710A | 1 × 25μl | 10ng/μl | View Product |
|
PowerPlex® ESX 17 Allelic Ladder Mix |
DG150B | 4 × 50μl | ||
WEN Internal Lane Standard 500 |
DG500A | 2 × 200μl | View Product | |
|
PowerPlex® ESX 17 10X Primer Pair Mix |
DK149B | 4 × 250μl | ||
|
PowerPlex® ESX 5X Master Mix |
DP102A | 4 × 500μl | ||
|
Water, Amplification Grade |
DW099A | 10 × 1,250μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
U.S. Pat. No. 9,139,868, European Pat. No. 2972229, Japanese Pat. No. 6367307 and other patents pending.
TMR-ET, CXR-ET and WEN dyes are proprietary.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
PowerPlex® ESX 17 System |
DC6720 | 1 × 400 reactions |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
U.S. Pat. No. 9,139,868, European Pat. No. 2972229, Japanese Pat. No. 6367307 and other patents pending.
TMR-ET, CXR-ET and WEN dyes are proprietary.
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
2800M Control DNA |
DD710A | 1 × 25μl | 10ng/μl | View Product |
|
PowerPlex® ESI 17 Pro Allelic Ladder Mix |
DG246B | 4 × 50μl | ||
WEN Internal Lane Standard 500 ESS |
DG510A | 2 × 200μl | View Product | |
|
PowerPlex® ESI 17 Pro 10X Primer Pair Mix |
DK210B | 4 × 250μl | ||
|
PowerPlex® ESI 5X Master Mix |
DP103A | 4 × 500μl | ||
|
Water, Amplification Grade |
DW099A | 10 × 1,250μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
U.S. Pat. No. 9,139,868, European Pat. No. 2972229, Japanese Pat. No. 6367307 and other patents pending.
TMR-ET, CXR-ET and WEN dyes are proprietary.
Resources
Software and Firmware
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