ReliaPrep™ RNA Clean-Up and Concentration System
Concentrate RNA from Large Volumes to ≤15µl
- Column-based method for rapid concentration and clean-up of RNA
- High binding capacity to accommodate large samples
- Small elution volumes provide highly concentrated samples and remove inhibitors
Catalog Number:
Size
Catalog Number: Z1071
Catalog Number: Z1072
Catalog Number: Z1073
Prepare RNA Samples for Downstream Applications
The ReliaPrep™ RNA Clean-Up and Concentration System was designed to reduce carryover of reagents and concentrate dilute samples in a simple, fast protocol. The system can process dilute samples or sample pools up to 300µl in a single column and elute them in 15µl of water or TE buffer. It maintains good recovery of RNA samples and ensures usefulness in downstream applications such as RT-qPCR, Northern blot analysis or RNA sequencing.
Recovery and Concentration of RNA from Dilute Solution using ReliaPrep™ RNA Clean-Up and Concentration System
Five micrograms of mouse liver RNA was suspended in 100, 150, 200 or 300µl of water, repurified using the standard protocol and eluted in 15µl of Nuclease-Free Water. Results shown demonstrate equivalent yield regardless of target RNA concentration. Average RNA yield was 79.8%.
One microgram of mouse liver RNA was suspended in 100, 150, 200 or 300µl of water, repurified using the standard protocol and eluted in 15µl of Nuclease-Free Water. Results shown demonstrate equivalent yield regardless of target RNA concentration. Average RNA yield was 74.9%.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130C | 1 × 10/pk | |
| ReliaPrep™ Minicolumns | A207A | 1 × 10/pack | |
Column Wash Solution (CWE) |
A254A | 1 × 1ml | |
|
Membrane Binding Solution |
A930A | 1 × 4ml | View Product |
|
Elution Tubes |
Z200A | 2 × 5/pk | |
|
RNA Wash Solution (RWA) |
Z309B | 1 × 11.8ml | View Product |
|
Nuclease-Free Water |
P119A | 1 × 1,250ÎĽl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Column Wash Solution (CWE) |
A254B | 1 × 5ml | |
|
Membrane Binding Solution |
A930B | 1 × 20ml | View Product |
|
RNA Wash Solution (RWA) |
Z309B | 1 × 11.8ml | View Product |
ReliaPrep™ Minicolumns |
A207B | 1 × 50/pack | |
Collection Tubes |
A130A | 1 × 50/pk | |
|
Elution Tubes |
Z200B | 2 × 25/pk | |
|
Nuclease-Free Water |
P119A | 1 × 1,250ÎĽl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Column Wash Solution (CWE) |
A254C | 1 × 24ml | |
|
Membrane Binding Solution |
A930B | 2 × 20ml | View Product |
|
Elution Tubes |
Z200C | 5 × 50/pk | |
|
RNA Wash Solution (RWA) |
Z3091 | 1 × 58.8ml | View Product |
ReliaPrep™ Minicolumns |
A207B | 5 × 50/pack | |
|
Collection Tubes |
A130B | 1 × 250/pk | |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
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