HaloTag® Ligand Building Blocks
Enables any Reporter, Protein or Nucleic Acid with Specified Functional Groups to be used with the HaloTag® Protein
- Image live or fixed mammalian cells expressing HaloTag® protein or protein fusions
- Analyze post-translational modification
- Isolate proteins and protein complexes
Catalog Number:
Choose a Ligand
Size
Catalog Number: P6711
Catalog Number: P6741
Catalog Number: P1691
Catalog Number: P6751
Ligand Building Blocks can carry a variety of functionalities, including fluorescent labels, affinity tags and attachments to a solid phase. The covalent bond forms rapidly under general physiological conditions, is highly specific and essentially irreversible. The HaloTag® Ligand Building Blocks allow researchers to apply the chloroalkane group that HaloTag® protein reacts with to any compound or surface with a compatible chemical group, creating endless possible applications.
The HaloTag® Succinimidyl Ester (O4) Ligand contains a reactive succinimidyl ester (SE) group connected to an alkyl chloride separated by three ethylene glycol repeats (O4). The HaloTag® Succinimidyl Ester (O4) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an amine, forming stable amide bond linkages. The ligand with functional group can then be used with the HaloTag® protein for any application of interest.
The HaloTag® Succinimidyl Ester (O2) Ligand contains a reactive succinimidyl ester (SE) group connected to an alkylchloride separated by an ethylene glycol repeat (O2). The HaloTag® Succinimidyl Ester (O2) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an amine, forming stable amide bond linkages (see figure). The ligand with functional group can then be used with the HaloTag® protein for any application of interest.
The HaloTag® Amine (O4) Ligand contains a reactive amine group connected to an alkyl chloride, separated by an ethylene glycol repeat (O4). The HaloTag® Amine (O4) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an activated carboxylic acid, sulfonyl halide or isocyanate. Examples of activated carboxylic acids are succinimidyl esters, STP esters, acid halides, and TFP esters. The ligand with functional group can then be used with the HaloTag® protein for any application of interest.
The HaloTag® Amine (O2) Ligand contains a reactive amine group connected to an alkylchloride, separated by an ethylene glycol repeat (O2). The HaloTag® Amine (O2) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an activated carboxylic acid, sulfonyl halide or isocyanate. Examples of activated carboxylic acids are succinimidyl esters (see figure), STP esters, acid halides and TFP esters. The ligand with functional group can then be used with the HaloTag® protein for any application of interest.
For more information, see the Protocols & Applications Guide.
- Hermanson, G.T. (1996) In: Bioconjugate Techniques, Academic Press, San Diego, CA, 139–40.
- Gretch, D.R., Suter, M. and Stinski, M.F. (1987) Anal. Biochem. 163, 270–77.
- Duthie, R.S. et al. (2002) Bioconjugate Chem. 13, 699–706.
- Hermanson, G.T. (1996) In: Bioconjugate Techniques, Academic Press, San Diego, CA, 137–46.
- Gee, K.R. et al. (1999) Tetrahedron Letters 40, 1471–4.
- Adamczyk, M. et al. (1997) Bioconjugate Chem. 8, 253–5.
- Hermanson, G.T. (1996) In: Bioconjugate Techniques, Academic Press, San Diego, CA, 146–8.
- Chen, J. and Selvin, P.R. (1999) Bioconjugate Chem. 10, 311–5.
- Ge, P. and Selvin, P.R. (2003) Bioconjugate Chem. 14, 870–76.
- Krokhin, O.V. et al. (2003) Biochemistry 42, 12950–59.
- Hermanson, G.T. (1996) In: Bioconjugate Techniques, Academic Press, San Diego, CA, 146–52.
Example reaction with HaloTag® Amine (O4) Ligand.
Protocols
Complete Protocol
Specifications
Catalog Number:
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only and may modify or link Promega HaloTag® ligand building blocks to Promega or customer-supplied moieties. No transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only and may modify or link Promega HaloTag® ligand building blocks to Promega or customer-supplied moieties. No transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only and may modify or link Promega HaloTag® ligand building blocks to Promega or customer-supplied moieties. No transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only and may modify or link Promega HaloTag® ligand building blocks to Promega or customer-supplied moieties. No transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
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