We will be conducting our annual physical inventory from March 11th through March 13th.

Orders received after 4pm CST on Tuesday, March 10th will be scheduled to ship as follows:

US Orders: Ship Saturday, March 14th for arrival on Monday, March 16th.
Canada Orders: Ship Monday, March 16th for arrival on Tuesday, March 17th.

Select items may ship earlier. For questions regarding the status of your order, contact custserv@promega.com.

Magne® Protein G and Magne® Protein A Beads

G7471_Magne--Protein-G-Beads--20--Slurry_3 G7472_Magne--Protein-G-Beads--20--Slurry_3 G7473_Magne--Protein-G-Beads--20--Slurry_3 G8781_Magne-Protein-A-Beads--20--Slurry_3 G8782_Magne--Protein-A-Beads--20--Slurry_3 G8783_Magne--Protein-A-Beads--20--Slurry_3

High Specificity, High Capacity Magnetic Affinity Beads for Antibody Capture

  • Binding capacities of >25mg/ml depending on antibody species, isotype
  • Minimize purification losses and increase sample throughput with exceptional magnetic properties

Catalog Number:

Choose Protein G or A Beads

Size

Catalog Number: G7471

Please Enquire

Add G7471 to Helix

Catalog Number: G7472

Please Enquire

Add G7472 to Helix

Catalog Number: G7473

Please Enquire

Catalog Number: G8781

Please Enquire

Add G8781 to Helix

Catalog Number: G8782

Please Enquire

Add G8782 to Helix

Catalog Number: G8783

Please Enquire


Overview
Protocols
Specifications
Resources
Related Products
Citations

Magnetic Protein A and G Antibody Purification

High-yield, robust purification of antibodies from serum, ascites and cell media.

(Note: Magne® Protein G and Magne® Protein A Beads are not recommended for use in immunoprecipitation (IP) or co-IP applications.)

Antibody Purified from Various Sample Types using the Magne® Protein A and Magne® Protein G Beads.

11208TA-W
Antibody was purified from 50μl of cell culture media (mouse IgG1), mouse ascites (IgG2a) and goat serum with 50μl of Magne® Protein A Beads (A) and Magne® Protein G Beads (G) using published protocols (Technical Manual #TM371). Samples were separated via SDS-PAGE by adding 1μl of starting material (SM) or 5μl of purified sample (A or G) and stained with Coomassie®-based stain

Schematic of Antibody Purification using Magne® Protein A Beads or Magne® Protein G Beads.

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Competitor Comparison for Magnetic Antibody Purification Resins

10751MA-W
Comparison of antibody yield using the Magne® Protein G Beads versus competitors' products from diverse sample types. *Delipidated human gamma globulin serum fraction was used as the source material.

Relative Binding Affinities of Protein A and Protein G for Different Antibody Species and Isotypes.

Species/ Subclass Protein A Protein G
Monoclonal
Human
IgG1 +++ +++
IgG2 +++ +++
IgG3 + +++
IgG4 +++ +++
Key: Strong (+++), Medium (++), Weak (+). Adapted from Akerström, B. et al. (1985) J. Immunol. 135(4), 2589-92.
Species/ Subclass Protein A Protein G
Monoclonal
Mouse
IgG1 + +++
IgG2a +++ +++
IgG2b ++ ++
IgG3 + ++
Species/ Subclass Protein A Protein G
Monoclonal
Rat
IgG1 + +
IgG2a + +++
IgG2b + ++
IgG2c + +
Species/ Subclass Protein A Protein G
Polyclonal
Rabbit +++ +++
Cow ++ +++
Goat + +++
Chicken + +
Human IgG +++ +++
Human IgM + +
Human IgD + +
Human IgA + +

Specifications

Catalog Number:

What's in the box?

Item Part # Size

Magne® Protein G Beads

G747A 1 × 1ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

 

What's in the box?

Item Part # Size

Magne® Protein G Beads

G747A 5 × 1ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

 

What's in the box?

Item Part # Size

Magne® Protein G Beads, 20% Slurry

G747B 1 × 50ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

 

What's in the box?

Item Part # Size

Magne® Protein A Beads, 20% Slurry

G878A 1 × 1ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

 

What's in the box?

Item Part # Size

Magne® Protein A Beads, 20% Slurry

G878A 5 × 1ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

 

What's in the box?

Item Part # Size

Magne® Protein A Beads, 20% Slurry

G878B 1 × 50ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

 

Resources

No related resources found

Product Citations

Recent publications that mention the use of this product.

View PubMed Citations