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Exonuclease III Protocol

Instructions for Use of Product(s)
M1811, M1815

Literature # 9PIM181

Exonuclease III (Exo III) has a double-strand specific, nonprocessive 3´to 5´ exo-deoxyribonuclease activity. The enzyme catalyzes the stepwise removal of mononucleotides starting from a 3´-OH at blunt ends, recessed ends and nicks; however, 3´-overhangs of >=4 bases are protected from Exo III activity.

Revised 11/20.

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