IL-6 Bioassay Technical Manual
Instructions for Use of Product(s)
JA2501, JA2505, JA3501, JA3505
Literature # TM593
The IL-6 Bioassay is a bioluminescent cell-based assay designed to measure IL-6 stimulation or inhibition using a response element as a readout. The IL-6 Bioassay Cells are provided in a thaw-and-use format, as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell propagation. The IL-6 Bioassay consists of human cells engineered to express the IL-6 receptor and a luciferase reporter driven by a response element (RE).When IL-6 binds, the IL-6R transduces intracellular signals resulting in luminescence. The bioluminescent signal is detected and quantified using Bio-Glo™ Luciferase Assay System and a standard luminometer, such as the GloMax® Discover System.
Summary of Changes
The following changes were made to the 7/25 revision of this document:
1. Updated text about the label in Section 3.
2. Updated the cover image and fonts.
3. Removed an expired patent statement and updated another statement.
4. Made miscellaneous text edits and updates.
Revised 7/25.